摘要
采用体外(in vitro)固体和液体培养玉米大斑病菌——大斑病长晨蠕孢(Helminthosporium turcicum),培养物经有机溶剂萃取后减压蒸发获得了Ht-粗毒素,最后用种子根伸长抑制,离体根冠细胞死亡和离体叶片致萎等生物测定法,对玉米大斑病菌的体外代谢产物进行了致病活性和毒性测定,筛选出乙腈等有机溶剂,可以用于Ht-毒素的萃取,为Ht-毒素的进一步提纯和结构分析奠定了基础。
Ht-toxin was extracted by singular or mixed organic solvents after solid and liquid culturing Helminthosporium turcicum in vitro, and pathogenic activity and toxicity of these extracts were assayed using root growth inhibition, isolated root cap cell death and isolated leaf soaking bioassays. Several best singular and mixed organic solvents have been screened for extracting Ht-toxin in vitro and this may lay foundation for its purification and structure analysis.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第2期73-77,共5页
Microbiology China
关键词
玉米
大斑病长蠕孢
真菌毒素
Helminthosporium turcicum
Mycotoxin
Bioassay
Extraction of Ht-toxin
Maize
