γ-亚麻酸高产菌株的选育及发酵产物的分离提取

BREEDING OF A HIGH-γ-LINOLENIC ACID YIELD PRODUCING STRAIN AND THE EXTRACTION OF FERMENTATION PRODUCT

以深黄被孢霉AS3.3410为出发菌株发醇产生γ-亚麻酸,其菌体得率为10%,油脂含量为27%,γ-亚麻酸含量为3.3%。经过紫外线诱变处理。得到变异株M_6,其菌体得率为25%,油脂含量为32.8%,γ-亚麻酸含量为8.84%。传代实验表明,M_6具有良好的遗传稳定性。经摇瓶发酵条件试验,选出了最佳培养基配方,以10升罐进行发酵,结果为:菌体得率29.3%,油脂含量44.7%,γ-亚麻酸含量9.44%。菌体油脂提取方法的研究表明,以乙醇和正己烷对湿菌体进行分步抽提效果较好。

Mortieralla isbellina (AS 3.3410) was used as the starting strain in the experiment. Through fermentation, the production rate of fungal body was 10%, content of lipid in fungal body was 27%, content of γ-Linolenic acid in lipid was 3.3%, When it was treated by U.V, mutant M_6 was obtained, its production rate of fungai body was 25%, lipid content was 32.8%, γ-linolenic acid content was 8.44%. Pass-generation test indicated that the heredity character of M_6 was stable.The optimum culture medium was obtained through culture condition test, and the result of fermentation in 10L tank showed that the production rate of fungal body was 29.3%, lipid content was 44.7%, GLA content was 9.44%.In the meantime, the method of extraction of fungai lipid was primarily studied, which indicated. that it is preferable to use a combination of ethanol and hexane to extract alternately.