菌种保存方法对冰核细菌冰核活性的影响

THE EFFECT OF PRESERVATION ON ICE NUCLEATION ACTIVITY OF INA BACTERIA

本文测定了5种保存方法及3种保存温度,对4株代表3个属4个种(变种)的冰核细菌冰棱活性的影响。结果表明,不同保存方法对冰核细菌的存活力和冰核活性存在着不同程度的影响,保存温度越高,对冰核活性的影响越大,不同菌种对保存的敏感性不同。所适合的保存方法也不同。真空冷冻干燥和灭菌水于-20℃冷冻保存对各菌株的存活力和冰核活性的影响都较小,适用于一般冰棱细菌的长期保存。而10%甘油冷冻保存则不适用于冰核细菌的保存。

The effect of 5 store methods at 3 different temperature on ice nucleation activity of INA bacteria were evaluated. The results showed that some store methods could decrease the ice nucleation activity. The effect increased as the temperature of increased. The effect on survival and on activity is different. Different strains had different susceptibility and adaptability. It was found that the freeze-drying method storing at -20℃ and freezing in sterile distilled water storing at -20℃ could be used for most INA bacteria. Freezing in 10% glycerin could not be used for the store of INA bacteria.