细菌透析袋表面培养法及其蛋白质产物的检测

BACTERIA CULTURE ON SURFACE OF DIALYTIC-BAGS AND ANALYZATION OF BACTERIA-PRODUCED PROTEIN

本文报告的细菌透析袋表面培养法,可使被检菌的浓度比普通培养法高10倍左右,并避免了培养基中高分子物质混入,以上清原液直接进行SDS-PAGE得到了清晰的带谱。对葡萄球菌产生的蛋白质带谱观察显示:不同菌种、菌株间带谱都有各自不同特征,各菌株的带谱重复性良好。本培养法在细菌蛋白质产物分析及临床细菌鉴定中是很有实用价值的。

This article reports a method of bacteria culture on the surface of dialytic-bags which can prevent macromolecules from penetrating in or out. Using this method the author cultured 8 strains of staphylococcus and from the external dialytic fluid samples of supernatant were collected and analyzed with SDS-PAGE. The result showed that the concentration of bacteria cultured with this surface method was about 10 times as high as that with usual method. The concentration of bacteria-produced protein in the supernatant was also high enouph to give a clear protein spectrum with the original sample without concentrating,and different supernatant from different species and/or strains gave different spectrum patterns. The reproducibility of the experiments with all strains of bacteria was very satisfying. The data indicate that this cultural method may be useful in analyzation of bacterial protein products and clinical identification of bacteria.