不同抗生素对甘薯遗传转化的影响

Effects of different antibiotics on genetic transformation of sweet potato

探讨了卡那霉素(Kan)、头孢霉素(Cef)、羧苄青霉素(Cn)3种抗生素对甘薯遗传转化植株生长的影响。确立了甘薯用农杆菌介导的遗传转化研究中抑菌剂和筛选剂的计量方案。甘薯腋芽对卡那霉素(Kan)敏感,甘薯叶片次之。当卡那霉素(Kan)为50mg/L时植株的生长基本停止,当卡那霉素(Kan)为70mg/L时,甘薯腋芽一周就变白,3～5d褐变死亡。甘薯叶片20d左右全变白,并慢慢死亡。头孢霉素(Cef)为200mg/L时对甘薯愈伤的分化有一定的促进作用。羧苄青霉素(Cn)对甘薯的愈伤的分化影响不太明显,但浓度超过400mg/L时对甘薯出愈有抑制作用。

The effect of three antibiotics on growth of sweetpatato,including Kanamycin (Kan),carbentcillin (Cn)and cefotaxime (Cef),was discussed in this article.A doses strategy of bacterium inhibition and selection.using agrobacter mediated genetic transformation was established.The results showed that sweet potato bud was very sensitive to Kan,and sweet potato leaf followed it .When the concentration of Kan was 50 mg/L,the growth of sweet potato was almost stop,When the concenttration of Kan was 70 mg/L,sweet potato bud would turn white,then it would turn browning and died in 3 to 5 days.The leaves of sweet potato turn entirely white about in 20 days.200 mg/L cef could accelerate the differentiation of sweet potato callus, while Cn had no apparent influence on it,but when Cn concentration was over 400 mg/L,the inhibiting effect was observed.