丙戊茶碱对吗啡耐受大鼠脊髓星型胶质细胞的影响

Effect of propentofylline on spinal astrocytes in morphine tolerant rats

目的 观察脊髓星型胶质细胞(AST)在吗啡耐受过程中的变化。方法 建立慢性吗啡耐受大鼠模型,用丙戊茶碱(propentofylline)进行干预,用免疫组织化学方法测定脊髓后角AST活性在吗啡耐受过程中的变化,用热辐射抬足法测定痛阈。结果 吗啡耐受形成过程中胶质纤维酸性蛋白(GFAP)免疫反应呈强阳性,丙戊茶碱有部分抗吗啡耐受作用,并且可以使吗啡耐受过程中GFAP免疫反应阳性产物减少。结论 吗啡耐受的机制可能涉及脊髓 AST的激活,丙戊茶碱可能通过抑制脊髓AST激活而具有部分抗吗啡耐受作用。

Objective To investigate the changes of the spinal astrocytes(AST) in the development of morphine tolerance in rats. Methods The chronic morphine tolerance rats model was established. The changes of the spinal astrocytes response to chronic morphine tolerance was examined by immunohistochemistry of glial fibrillary acidic protein(GFAP).The pain threshold was measured using the conventioned paw withdrawal test. Results The morphine tolerance led to significant increase in GFAP immunostaining density in the spinal cord.Propentofylline administrated with morphine could block the development of morphine tolerance and the increase of GFAP immunostaining density. Conclusion The activity of the ASTs in spinal cord may be involved in the mechanism of morphine tolerance. Propentofylline may partly block the development of morphine tolerance by inhibiting the activity of ASTs.