氯胺酮麻醉引起-fos基因在大鼠大脑皮质的表达

The effect of ketamine on c-fos gene expression in rat cerebral cortex

目的检测氯胺酮对大鼠大脑皮质c-fos基因表达的影响,探讨氯胺酮对中枢神经系统作用的机制.方法将48只Wistar大鼠随机分为六组,即生理盐水组,给药后15、30、60、120和180 min组,每组8只.腹腔注射氯胺酮100 mg/kg后,分别于给药前(生理盐水组)和给药后15、30、60、120、180min提取大脑皮质总RNA量,经过逆转录多聚酶链反应,扩增出cDNA产物.用成像分析系统计算曲线下面积,与内参照比较表示各时点的c-fox mRNA水平.免疫组织化学方法检测各组Fos蛋白阳性细胞率.结果氯胺酮注射后引起大脑皮质c-fos mRNA表达增高,其高峰在30～60min之间,3 h后回至基线水平,且与行为学变化呈时间相关性.氯胺酮注射后1 h引起大脑皮质Fos阳性细胞率明显增高(P＜0.05).结论c-fos基因参与了氯胺酮所致精神症状的分子基因机制.

Objective To determine profiles of c-fos gene expression in rat cerebral cortex after administion of ketamine.Methods At 0, 15,30,60,120,180min after given ketamine100mg /kg intraperitoneally ,the rats were sacrified. Total cellular RNA of the cerebral cortex was extracted. The semiquantitative reverse transcription- polymerase chain reaction method was used to determine cDNA amplification products.Gama -actin mRNA was served as an internal control. Densities of DNA bands were quantified using image analysis system. Fos protein expression was observed by immuno-histechemical technique. Results C-fos mRNA increased at 15 min after 100mg/kg ketamine , peaked at 30 min and 60 min and decreased at hours after injection. At 180 min after injection, c-fos mRNA recovered to base level. After 1h after injection ,ketamine induced marked Fos protein expression (P<0.05). Conclusion C-fos gene involves in molecular modulation of psychological reaction induced by ketamine.