应用遗传毒理短期测试研究氰戊菊酯致突变性

Study on the mutagenicity of fenvalerate with short-term tests

报道的氰戊菊酯致突变性结果不一,为此用多种方法研究了氰戊菊酯标准品和工业品的致突变性。结果发现:小鼠骨髓多染红细胞微核试验阴性,但见雄性较大剂量时有随剂量增高趋势。小鼠睾丸初级精母细胞染色体数目畸变阴性,染色体结构畸变阳性。人体外周血淋巴细胞SCE频率虽与阴性对照比差别有显著意义,但无剂量反应关系,为阴性。人体外周血淋巴细胞UDS 试验,氰戊菊酯工业品油剂及日产粉剂呈较强阳性;标准品阳性较弱。综合上述材料,认为氰戊菊酯标准品是一弱阳性致突变剂,其工业品致突变性增强,可见杂质起了重要作用。因此,减少我国工业品中之杂质、提高纯度,可望降低其致突变性能。

The mutagenicity of fenvalerate was studied with four short-term genotoxic tests. The res-ults showed that (1) fenvalerate did not induce micronulei in polychromatic erythrocytes ofmouse bone marrow; (2) fenvalerate could induce the structure aberrations, but not the num-erical aberrations in primary spermatogenic cells of mice; (3) sister chromatid exchangefrequencies were slightly but significantly increased in cultured human peripheral blood lymp-ocytes in vitro; and (4) fenvalerate enhanced the unscheduled DNA synthesis of human pe-ripheral blood lymphocytes iu vitro. These results indicated that fenvalerate is a weak muta-en and this effect was more prominant by technical product which could contain unkown mu-tagenic impurities. Therefore, the purity of the product of fenvalerate should be improved toeliminate the mutagenic potential.