大鼠气管上皮细胞体内-体外转化模型的研究

In Vivo-In Vitro transformation test of rat tracheal epithelial cells induced by intratrachea\ tnstillation of carcinogens

本研究建立了大鼠气管上皮细胞体内-体外转化模型。大鼠气管内滴注苯并(a)芘,三天后处死大鼠,消化气管上皮细胞,接种于无血清完全培养基。细胞形成集落后,换为选择培养基继续培养五周,统计转化率。结果显示,25mg/kg和50mg/kg的苯并(a)芘可诱导大鼠气管上皮细胞转化及微核增加。用同样方法研究了煤焦沥青提取物,结果表明,剂量为8mg/kg和25mg/kg的煤焦沥青提取物能明显诱导大鼠气管上皮细胞转化。

An in vivo-in vitro transformation system was established using primary rat tracheal ep-ithelial (RTE) cells. Rats were exposed to benzo (a) pyrene (BP,25～50mg/kg.bw) or coal tarpitch extract (CTP,8～25mg/kg.bw) by intra tracheal instillation. RTE cells were isolated 3days later, and cultured in serumfree F12 medium supplemented with hormones and growthfactors. One week after plating, RTE cells were cultured in selective medium with serum for5 weeks.The results showed that transformed colonies were induced by BP or CTP. Increasein micronucleus fregnency was also obseiyed in EP treated cells. Valvable thing is that veryearly stages of carcinogenesis can be detected using this system in the primary epithelial cellsfrom the tissue exposed in vivo.The in vivo-in vitro transformation sustem seems particularlysuitable for presere-ening potential carcinogens.