摘要
运用冷休克、热休克、静水压三种方法抑制金鱼受精卵第二次减数分裂,诱导金鱼三倍体.结果表明:金鱼三倍体诱导率明显受压力、处理温度、处理起始时间以及处理持续时间等因子的影响;在22℃水温下,受精8min后,在0℃冷休克条件下,持续处理14min的三倍体诱导率可达25.5%;受精10min后,在39℃热休克条件下,持续处理1min,三倍体诱导率可达100%;受精4min后,在550kg cm2静水压休克条件下,持续处理3min三倍体诱导率可达28.6%.还对提高金鱼三倍体诱导率和孵化率的方法进行了探讨.
Cold shock,heat shock and hydraulic pressure were used to restrain the second meiosis of the fertilized gold fish eggs and to induce gold fish triploid.It turned out that the gold fish triploid induction rate was obviously affected by the pressure,the treatment temperature,the beginning and continuing treatment time and so on.The triploid induction rate could reach 25 % 8 minutes after fertilization with 0 ℃ cold shock when the continuing treatment time being 14 minutes in 22 ℃ water,and 10 minutes after fertilization with (39 ℃) heat shock when the continuing treatment time being 1 minute ,it could get to 100 %.It could also be 28.6 % 4 minutes after fertilization,with 550 kg/cm^2hydraulic pressure shock when the continuing treatment time being 3 minutes.How to increase the rate of the gold fish triploid induction and hatch was also discussed.
出处
《集美大学学报（自然科学版）》
CAS
北大核心
2004年第4期305-309,共5页
Journal of Jimei University：Natural Science
基金
2003年福建省科技型中小企业技术创新资金资助项目(2003C065W)
关键词
金鱼
三倍体
冷休克
热休克
静水压休克
gold fish
triploid
cold shock
heat shock
hydraulic pressure shock
