BTH对新疆甜瓜过氧化物酶的系统诱导作用

The attractive action of BTH on Xinjiang melon peroxidase

采用3种浓度(25,50,100μg·ml-1)的BTH对耐病品种皇后和感病品种早金的第一片真叶进行诱导处理后,经诱导后不同时间测定第一片和第二片真叶的过氧化物酶(POD)活性,结果表明:BTH的三种不同的浓度处理均能诱导出耐病品种皇后第一片和第二片真叶的过氧化物酶(POD)活性的升高,与空白差异显著,以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的为最低。第一片真叶的酶活性高峰为诱导处理后的第7天和第16天,第二片真叶的酶活性高峰为诱导处理后的第16天。BTH的三种不同的浓度处理也能诱导出感病品种早金第一片和第二片真叶的过氧化物酶(POD)活性的升高,但与空白差异不显著。以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的最低。证明了甜瓜抗病性与甜瓜植株过氧化物酶活力呈正相关。BTH诱导酶活性的效应可以通过植物的输导组织,传导给其它未经处理的甜瓜组织,诱导其它组织中的过氧化物酶(POD)活性的升高。以25μg·ml-1BTH诱导效果最好。

In this thesis, the attractive action of BTH on Xinjiang melon peroxidase was studied. Three concentrations of BTH are 25 μg·ml^(-1),50 μg·ml^(-1),100 μg·ml^(-1), which treated Early Golden Queen and Queen. The first and second leaf of melon were assayed by the peroxidase activity after the treatment with BTH. It was confirmed that perixidase activity has direct relation to melon' strain. At the same time, BTH can induce higher peroxidase activity and this conductivity can transmit from the first leaf to the second leaf after 6 days of the first melon enzyme perk on the Queen, which was affected by significantly, and the best concentration is (25 μg·ml^(-1).)At the same time, the same result was got on the Early Golden Queen, but this effect was not significant. But the time of conductivity was different from different resistance cultivars. This experiment showed that the peroxidase activity in the tissue of melon which did not spray inducer was induced, the domino effect can be transferred by transfer tissue.