摘要
利用重叠延伸PCR技术将K88ac STⅡ融合基因克隆于T载体上,将重组基因质粒转化到受体菌DH10B中,蓝白斑筛选阳性菌落,通过PCR、NcoI/XcoI酶切后测序,与genbank报道的K88ac结构基因序列进行比对,证明所克隆的目的片段为K88ac STII融合基因。
K88ac-STⅡ fused gene amplified by overlap extention PCR was cloned into T vector. Recombinant plasmid was transformed to E.coli DH10B.The positive recombinant clone was selected on Amp/IPTG/X-Gel agar plate and characterized by NcoI/XcoI enzyme digestion and PCR.Analising object gene/amino sequences,we drew a conclusion that the sequence of K88ac-STII fused gene is correct.
出处
《新疆农业大学学报》
CAS
2004年第4期63-66,共4页
Journal of Xinjiang Agricultural University
