SARS-CoV感染康复者血清对豚鼠肺、脾脏功能的影响

The effect of convalescent sera of SARS patients on the lung of guinea pigs

目的:为了探讨SARS CoV感染患者急性病理损伤的免疫反应机制。方法:应用SARS CoV感染 康复者血清24h内多次注射豚鼠体内,观察肺脏损伤及血液气体变化。结果:动物在第3次注射康复者血清后出 现急性严重呼吸困难,血氧明显降低、二氧化碳明显升高,肺组织病理显示肺组织大量炎细胞浸润,脾组织白髓淋 巴细胞减少,而用正常人血清注射动物后未出现类似变化。结论:给豚鼠多次注射SARS CoV感染康复者血清可导 致急性肺损伤。

Obectives:To aid understanding of the pathogenesis of severe acute respiratory syndrome (SARS), the novel infectious disease with global impact, and the relation to current therapy. Methods:the Guinea pig was injected with convalescent sera for several times in 24 hours, which were obtained from confirmed SARS patients on average 40 days. artery blood gas was measured and pathological samples were examined by light microscopy.Results:the Guinea pig showed severe acute dyspnea after the third time injection with convalescent serum of SARS patient. partial pressure of oxygen (PO 2) and oxygen saturation were signigicantly decreased while the partial pressure of carbon dioxide was improved, and a pronounced inflammatory-cell infiltrate was seen in the alveoli and the interstitium of the lung pathological samples. Conclusion:Injecting convalescent sera of SARS patients may cause acute damage on the lung of guinea pig.