瘦素受体基因Pro1019Pro变异与单纯性肥胖的关系

Variation of leptin receptor gene pro1019pro and their relation to simple obesity in childhood

目的 探讨瘦素受体基因第 2 0号外显子 30 5 7位Pro10 19Pro基因变异与儿童单纯性肥胖之间的关系。方法 应用聚合酶链反应—限制性酶切片断长度多态性 (PCR -RFLP)方法测定瘦素受体基因Pro10 19Pro的基因变异频率 ,同时测血脂、身高、体重。结果 瘦素受体基因Pro10 19Pro(G→A)基因变异频率在肥胖组与正常对照组间差异无显著意义 (P >0 .0 5 )。血脂水平与体重指数 (BMI)在肥胖组与正常对照组间差异有显著意义 (P <0 .0 5 ) ;肥胖组中及正常对照组中GG基因型与AA基因型间BMI值与血脂水平均差异无显著意义 (P >0 .0 5 )。结论 瘦素受体基因第 2 0号外显子 30 5 7位Pro10 19Pro变异在儿童单纯性肥胖的发病机制中无重要作用 。

Objective To investigate the possible relationship between the leptin receptor(lepr) gene exon 20 nucleotide 3057 Pro1019Pro(G→A) variation and simple obesity in childhood.Methods Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) were used to detect the variation frequency of leptin receptor gene nucleotide 3057(G→A) transition. The blood lipid were detected with enzymic method.Simultaneusly,we detected the body height and body weight of all subjects.Results There were no significant differences between the obese group and the non-obese group about the variation frequency at the lepr gene 3057 Pro1019Pro (G→A) variation (P>0.05).There were significant differences between obese group and non-obese group about the serum levels of TG、TC、HDL-c and LDL-c and the BMI.There were no significant differences between GG phenotype and AA phenotype about the serum levels and the BMI in obese group and in non-obese group either. Conclusion It is unlikely that Leptin receptor gene Pro1019Pro variation may contribute to the pathogenesis of simple obesity in childhood.The gene variation had no effect on the blood lipid and BMI.