浙江省局部地区2002～2003年流行的无菌性脑膜脑炎的病原学检测与分析

Detection and Analysis of the Pathogen of Aseptic Meningitis Circulated in Zhejiang Province During 2002-2003

浙江省局部地区 2 0 0 2～ 2 0 0 3年多次出现无菌性脑膜脑炎的流行。为对疫情进行病原学检测与分析 ,采集了临床标本 ,采用HEp 2、RD、Vero等细胞分离病毒 ,并用肠道病毒通用引物进行特异性逆转录 聚合酶链反应 (RT PCR)扩增。结果显示 :5 1份脑脊液标本中分离出埃可病毒 30型 (ECHO3 0 ) 5株 ,柯萨奇病毒B组 5型 (Coxsack ievirusB5,Cox B5) 1株 ;6 8份粪便标本中分离出ECHO3 0 34株 ,Cox B51株。RT PCR的结果与上述病毒分离结果相一致。此外 ,对采集的 1 5例患者的急性期、恢复期双份血清测定了特异性中和抗体 ,其中 1 3例患者抗体呈≥ 4倍增长。证实引起浙江省局部地区 2 0 0 2～ 2 0 0 3年无菌性脑膜脑炎的主要病原为ECHO3 0 。

During 2002-2003 the aseptic meningitis epidemic occurred for many times in some local areas of Zhejiang Province. To detect and analyze the pathogen,we collected clinical samples and isolated viruses using Hep-2,RD, and Vero cell lines,then the viruses were amplified by specific RT-PCR with enterovirus universal primer.The results showed that 5 strains of ECHO 30 and 1 strain of Cox.B 5 were isolated from 51 cerebrospinal fluid(CSF)sample, 34 strains of ECHO 30 and 1 strain of Cox.B 5 were isolated from 68 stool samples.The results of PT-PCR were consistent with the above virus isolation results.Additionally, we detected the specific neutralized antibody in double sera collected during acute and convalescence stages from 15 patients,the antibody showed that ≥4 folds were increasing in 13 patients.It demonstrated that during 2002-2003 the main pathogen of aseptic meningitis circulating in some local areas of Zhejiang Province was ECHO 30.