中药对股骨头缺血坏死修复过程中血管内皮生长因子基因表达的影响

Effects of Danxian Kanggu capsule on the vascular endothelial growth factor expression during the repair of avascular necrosis of femoral head

目的探讨具有活血补肾作用的中药丹仙康骨胶囊对股骨头缺血坏死修复过程中血管内皮生长因子(VEGF)基因表达的影响。方法采用手术方法制作兔股骨头缺血坏死模型,应用逆转录聚合酶链反应(RTPCR)、免疫组化、微血管灌注及组织形态学方法观察中药丹仙康骨胶囊治疗兔股骨头坏死过程中血管内皮生长因子基因(VEGFmRNA)表达、血管生成及骨组织修复情况。结果股骨头坏死模型组4周时可见有坏死骨周围VEGFmRNA表达呈阳性,但随着时间的推移而减少,至8、12周时VEGFmRNA表达均为阴性,再生血管密度低,只见少量间充质细胞增生及新骨形成;丹仙康骨胶囊治疗组至8、12周时VEGFmRNA表达呈强阳性,并可见明显血管增生,血管密度显著增高,大量新骨形成。结论活血补肾的丹仙康骨胶囊能促进股骨头缺血坏死修复过程中VEGFmRNA的表达、血管的再生和新骨的形成。

Objective:To investigate the effects of Danxian Kanggu(DXKG)capsules extracted from Chinese drugs on the vascular endothelial growth factor expression during the repair of avascular necrosis of femoral head and explore the mechanism of anti-osteonecrosis of DXKG capsules.Methods:The model of avascular necrosis of femoral head was producted by operation on rabbit's femoral neck.VEGFmRNA expression were measured by both RT-PCR and immunohistochemistry.Histological study and microvascular perfusion were used for examination of osteogenisis and angiogenisis.Results:Compared with model group,DXKG group showed follow changes obviously:vascular endothelial growth factor expression significantly increased;Area dyed by ink increased;Numbers of empty bone lacuna decreased;Fibroblasts multiplicated in the margin of marrow;Osteoblasts multiplicated around the necrosis of trabecula.Conclusion:The anti-osteonecrosis effect of DXKG capsule may be passing through its increasable effect on vascular endothelial growth factor expression and production in osteoblats.