广东高州普通野生稻(Oryza rufipogon Griff)的遗传多样性和居群遗传分化研究

Genetic Diversity and Differentiation of Oryza rufipogon Populations in Gaozhou

利用 30对SSR引物对广东高州普通野生稻 3个群体进行了遗传多样性分析和居群遗传分化研究。结果表明 ,30对引物中只有 2 0对表现出多态 ,多态位点比率P为 6 6 7% ;在 2 0个多态位点中共检测出 81个等位变异 ,平均等位变异数(Ap)为 4 0 5个 ;3个群体总的遗传多样性 (Ht)为 0 6 1,其中 ,居群内的遗传多样性为居群间的遗传多样性的 3倍多 ,说明总的遗传多样性主要来自居群内 ;虽然居群间的遗传分化系数 (GST)较低 ,仅为 0 2 4 2 7,但当遗传相似系数临界值增加时 ,3个群体在聚类图上相对独立 ,说明 3个群体既存在着高度的遗传相似性 ,又具有一定程度的遗传分化 ,可以作为 3个居群进行原生境保护。

To study the genetic diversity and differentiation of the three populations of Oryza rufipogon in Gaozhou, Guangdong Province, 30 pairs of SSR primers were used. The results indicated that: i) Only 20 pairs of SSR primers were polymorphic and 77 alleles were detected. The percentage of polymorphic loci (P) and the mean number of alleles per locus (Ap) were 66 7% and 4 05 respectively. ii) The total gene diversity of the three populations was 0 61, among which more than 75% was from the gene diversity within populations. iii) Though the coefficient of gene differentiation (G ST) was 0 2427, meaning that the genetic variability among populations was low, the dendrogram of the samples from all populations showed that the three populations were relatively separated with the coefficient increasing. Therefore, the genetic differentiation of the three populations definitely occurred but not obviously.