离子缔合型胆红素阴离子选择电极的研制

PREPARATION AND STUDY OF THE IONIC ASSOCIATION SELECTIVE ELECTRODE FOR BILIRUBIN ANION

用三庚基十二烷基铵盐与胆红素的离于缔合物为活性物质,制成胆红素阴离子选择电极。电极的响应斜率与试液pH值有关,在pH为8.3时对胆红素一价阴离子具有能斯特响应。

A bilirubin anion selective electrode prepared with associated substance of triheptyldodecylammonium iodide and bilirubin has been described. The electrode exhibits a Nernstian response to bilirubin at pH 8.3 with a slope of 58 mV/pe and a linear range of 1×10^(-6) to 5×10^(-4) mol·L^(-1) bilirubin. The response slope is related to pH of testing solution. Benzoate, citrate, sulfonate, cystine, thiamine, glucose and common inorganic anions do not interfere the determination for bilirubin. The extraction constant of associated substance was measured.