美味猕猴桃子叶愈伤组织的原生质体培养和再生植株

CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS

从B_5和NN-69培养基(含1mg/L 2,4-D)上分别选出美味猕猴桃子叶愈伤组织系A_(11)B_2和A_(16)N_1。在B_5原生质体培养基中,A_(11)B_2的原生质体再生细胞形成小细胞团;在NN-69原生质体培养基中,A_(16)N_1的原生质体再生细胞能持续分裂形成愈伤组织。经过分步诱导再生,获得A_(16)N_1原生质体再生植株。

Callus lines of A_(11)B_2 and A_(16)N_1 were selected from cotyledon cultures on B_5 and NN-69 media containing 1mg/L 2,4-D, respectively. When protoplasts isolated from callus line A_(11)B_2 were cultured on the modified B_(?) medium, cell division was arrested at the stage of cell clusters. However, when protoplasts isolated from callus line A_(16)N_1 were cultured on the modified NN-69 medium, they had divided to form cell groups, then developed into callus, from which plants were regenerated by the method of the stepwise induction of organgenesis.