光学显微镜的最新发展

The Latest Development of Optical Microscopy

光学显微镜技术近年已有革命性发展，应用最新测量技术和最新数据处理技术，已由普通的二维成像发展为三维立体成像．其横向和纵向分辨率有了很大提高．重点介绍了共焦扫描光学显微镜，近场扫描光学显微镜及微分扫描光学显微镜等典型的最新技术的原理，潜力及应用。

The revolutionary achievement in optical microscopy has been made recently. Optical microscopy is rapidly developed beyond the traditional two-dimensional imagery into the reproduction of three-dimensional structures by using the latest measuring techniques and data processing technologies. The lateral and vertical resolutions of the observation system have been greatly improved. The principls and potentiality for applications related to the confocal laser scanning microscopy, near-field scanning optical microscopy and differential scanning optical microscopy are introduced in this paper.