摘要
本文报道了我们用联苯胺以及联苯胺衍生偶氮染料刚果红,依文思蓝经口染毒大鼠后用^(32)P后标记方法对其肝脏中的DNA加合物的检测结果,3种物质均检出1种DNA加合物,其含量分别为18.17,1.03,1.74μmol/kgDNA。结果表明联苯胺衍生偶氮染料经偶氮还原产生的联苯胺或联苯胺衍生物,能同联苯胺样在大鼠肝内形成加合物,这种DNA加合物很可能是接触联苯胺衍生偶氮染料的人群膀胱癌发病高的原因。
DNA in the livers of rats was isolated 24 h af-ter oral administration of benzidine, Congo red andEvan's blue, DNA adducts in the livers of ratswere investigated by a ^(32)P-postlabeling assay. Thelevel of DNA adducts in the livers of rats after oraladministration of benzidine, congo red andEvan'blue were 18. 17, 1. 03 and 1. 74μmol/kgDNA respectively. The results showed that themetabolites of the benzidine derivative azo dyes canform adducts with DNA in the livers of rats.These DNA adducts probably are one of the rea-sons that the incidence of the bladder cancer rosein the people group who are only exposed to benzi-dine derivative azo dyes.
出处
《西安医科大学学报》
CSCD
1993年第1期17-20,共4页
Journal of Xi'an Medical University(Chinese)
基金
国家自然科学基金
关键词
致癌物
联苯胺
DNA
加合物
carcinogenicity
benzidine
DNA adducts
^(32)P-postlabeling assay
