沙棘油中α-生育酚含量的反相高效液相层析分析法

ANALYSIS OF α-TOCOPHEROL CONTENTS IN SEA-BUCKTHORN OIL BY REVERSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

为了控制沙棘油的质量,建立了一种反相高效液相层析法(RP—HPLC)测定沙棘油中α—生育酚的含量。沙棘油样品皂化后,用乙醚提取,浓缩乙醚提取液,残渣溶解于无水乙醇中注入液相层析仪。层析条件:ZORBAX柱(4.6×150mm)甲醇作流动相(1ml/min),紫外检测波长,280nm。测定了陕西永寿产沙棘种子油和果油各3批,α—生育酚的平均含量分别为989mg/kg,1754mg/kg。方法回收率98.6%(种子油),102.2%(果油),变异系数小于5.44%。

For controlling the quality of sea buckthornoil, a reversed phase-high performance liquid chro-matography (RP-HPLC) method was establishedto determine the a-Tocopherol contents. Aftersaponated, the sea buckthorn oil samples were ex-tracted by ethyl ether. The extraction liquid wascondensed and tben dissolved in anhydreousethanol for liquid chromatographic analysis. Chro-matographic conditions were as follows: ZORBAXODS column (4. 6 × 150 mm); methanol as mobilephase (1. 0 ml/min); the wavelength of UV detec-tor: 280 nm. Three branches of sea buckthornseed oil and three branches of fruit oil which wereproduced by Yongshou County, Shaanxi Provincewere determined. The average contents of a-Toco-pherol were 989 mg/kg, and 1745 mg/kg, respec-tively. The recoveries of this method were 98. 6%(for seed oil), 102. 2% (for fruit oil) with CV lessthan 5. 44%.