β-环糊精键合相的制备及其在液相色谱中的应用

Preparation and Application of Stable β-Cyclodextrin Bonded Stationary Phases in HPLC

环糊精(Cyclodextrin,简称CD),即由D(+)-萄葡糖单元构成的环状低聚糖类化合物,能有选择地和许多物质形成包复物(Inclusion complexes),含有CD的固定相已广泛地被用于分离科学中,近年来,高效液相色谱CD键合相的制备及其应用在国外引起广泛的重视,本文报道β-CD键合相的一种新制备法及其在HPLC中的应用。

This paper reports the preparation and application of a stable 'p-cyclodextrin (β-CD) bonded stationary phase with high chromatogra-phic selectivity. Much higher loading amounts of (β-CD on the stationary phase can be obtained by the method developed in our lab. in which 3-glycidoxy-propyl trimethoxysilane firstly reacted with β-CD and then let the resulting si lane react with YWG80-5μ silica gel.By using the β-CD column, we have achieved separation of some difficult-to-resolve compounds, such as structural isomer and even epimer and enantiomer. It is the first time to obtain the direct resolution of the acidic moietes of fenvalerate and flucythrinate which are the common pyrethroid insecticides. In addition, resolution of (±)-norgestrel epimers was obtained on the acetylated β-CD bonded phase which shows different chromatographic selectivity from that of β-CD bonded phase.