放疗对肺癌患者血清凋亡细胞核DNA片段化的影响

Effect of Radiotherapy on Serum Apoptosis Nucleolus DNA Gragmentation in Patients with Lung Cancer

目的研究放疗对肺癌血清凋亡细胞核DNA片段化的影响。方法肺癌患者108例(男77例,女31例),平均55岁,全部经病理组织学证实。TNM分期为期11例,期35例,期62例。健康者25例(男18例,女7例)作对照,平均52岁。应用酶联免疫吸附分析法测定血清凋亡细胞核DNA片段化指数。对患者肺癌细胞进行常规培养并进行免疫组织化学分析以观察细胞凋亡现象。结果肺癌患者血清凋亡细胞核DNA片段化指数明显高于健康对照(0.98±0.77vs0.62±0.26,P<0.01),男女患者间和TNM分期不同无显著差异,但放疗有效后明显升高(n=49,0.99±0.69vs1.64±0.79,P<0.01);肺癌细胞培养可见细胞凋亡现象。结论肺癌患者血清凋亡细胞核DNA片段化指数明显高于健康对照,且放疗有效后明显升高,肺癌患者癌细胞培养可见细胞凋亡现象。

Objective:To explore the effect of radiotherapy on serum apoptosis nucleolus DNA gragmentation in patients with lung cancer.Methods:108 patients with lung cancer (male 77, female 31, mean age 55 years) diagnosed by histopathology containing Ⅱstage (n=11), Ⅲ stage (n=35) and Ⅳ stage (n=62) with TNM, and 25 health people (mmale18, female 7, mean age 52 years) as control were enrolled in the study. DNA gragmentation index numbers of serum apoptosis nucleolus were determined by ELISA.Apoptosis phenomenons were observed by immunohistochemistry by conventional culture of lung cancer cells.Results:Index number of DNA gragmentation of serum apoptosis nucleolus in patients with lung cancer were higher than that in health control (0.98 ± 0.77 vs 0.62 ±0.26,P<0.01), and no significant deviation between gender and TNM.But after chemotherapy, index number of DNA gragmentation of serum apoptosis nucleolus in patients with lung cancer were significantly higher than before-chemotherapy (n=49,0.99±0.69 vs 1.64±0.79,P<0.01). Apoptosis phenomenons could be observed in conventional culture lung cancer cells.Conclusion:Apoptosis phenomenons could be observed in conventional culture lung cancer cells. Index numbers of DNA gragmentation of serum apoptosis nucleolus in patients with lung cancer were higher than that in health control, and should increase after-radiotherapy.