摘要
生物体细胞中的游离核苷酸具有极其重要的生理功能,若干年来对它们的研究一直相当活跃。近几年,国外有人用离子交换色谱分离分析植物种子、叶片、块茎等组织中的核苷酸或核苷酸糖,取得了令人满意的结果。本文采用反相色谱,利用单核苷酸在结构和在不同pH值范围其pK值的差异,通过控制pH实现它们之间的分离。初步实验表明,在绿豆芽的细胞中,可能至少存在5′-UMP等若干种游离核苷酸。
A reversed-phase liquid chromatographic method for the analysis of free nucleotides (uri-dine-5'-monophosphate etc.) from sprout of lentil is described- It has been shown that some free nuceleo tides such as 5'-UMP, 5'-GMP and 5'-AMP exist in the cells of sprout of lentil.The method for the treatment of sprout of lentil and the optimum chromatographic conditions for separation of nuceleotides are discussed.
出处
《色谱》
CAS
CSCD
北大核心
1989年第2期108-110,共3页
Chinese Journal of Chromatography