甘草花药培养中愈伤组织诱导和芽再生植株

Calli Induction and Plantlet Regeneration in Anther Culture of Glycyrrhiza uralensis

用甘草单核靠边期花药离体培养,在多种培养基上诱导出傅愈伤组织。高浓度蔗糖有利于雄核发育,愈伤组织染色体数8,证明其为单倍体。经转代培养后分化出芽、再生植株。

Pollen calli were induced by sorts of medium from vitroculture of anther in which microspores were in latter stage. High concentration of sucrose is favourable for the male nuclear development. There were 8 chromosome in the calli cells,proving to be monoploid. Then,regeneration bud and planlet were formed by successive transfer culture.