条锈病菌侵染小麦不同时期过氧化物酶同工酶的变化

CHANGES OF PEROXIDASE ISOENZYME AT DIFFERENT STAGES OF WHEAT CAUSED BY WHEAT YELLOW STRIPE RUST

对抗条锈性不同的小麦品种和毒力不同的条锈菌小种互相作用过程中过氧化物酶(POD)同工酶的变化进行了研究。结果表明:条锈菌在入侵期(接菌48h)感病品种酶带一致,免疫品种酶带发生明显的变化,慢锈品种在Rf值0.60处增加1条酶带。酶活免疫品种高于感病品种(p<0.05);在发病期(接种20天)感病品种酶带发生了较大的变化,但免疫品种酶带稳定,慢锈品种在Rf值0.85处增加2条酶带。免疫品种酶活性比感病品种显著增强(p<0.01)。

This paper dealt with changes of peroxidase (POD) isoenayme in the interaction process between various resistant varieties to wheat yellow stripe rust and various virulent rust races. Results showed that during infectipon peroid (after 48 hours inoculation), susceptible variety had the same pattern of POD bands, immune variety had various bands and slowrusting one had an extra band at 0.60 value of Rf. POD activity in immune variety was higher than that in susceptible one (p<0.05). During disease occuring peroid (after 20 days inoculation), there was more variability of POD bands in susceptible variety, stability in immune variety and two extra bands at 0.85 value of Rf in slowrusting two POD activity in immune variety was higher than that in susceptible one (p<0.01).