高效液相色谱法同时测定饮料中的抗坏血酸、糖精、苯甲酸和山梨酸

Simultaneous Determination of Ascorbic Acid, Sac- charine, Benzole Acid and Sorbic Acid in Beverages by High Performance Liquid Chromatography

抗坏血酸、糖精、苯甲酸和山梨酸是饮料中常用的添加剂,后三种组分的HPLC法同时测定已有报道。本文拟定了可同时在七分钟内定量测定以上四种组分的HPLC法,该法具有快速、灵敏,准确等优点,四种组分的回收率均在90％以上,七次重复进样的变异系数均小于2％。 实验部分 (一)仪器及试剂 日本岛津LC-4A高效液相色谱仪,SPD-2AS型紫外检测器,C-R2AX数据处理系统。

A quantitative method is proposed for deter-mining the title co/npoauds simultaneously inbeverbages by HPLC, These components were separated on a Zorbax ODS column by using methanol/0.5% KH2PO4 (30:70) (pH=6) as mobile phase and detected by UV-254, The coefficients of variation of the four components for 7 replicas were all less than 2%, Therecoveries of the four components added to theblank beverages ranged from 90% to100%.