高效液相色谱法测定人体脑脊液和血液中的氯霉素含量

High Performance Liquid Chromatographic Deter- mination of Chloramphenicol in Human Plasma and Cerebrosfina! Fluid

氯霉素是一种高效、广谱抗生素。它能有效地渗入脑脊液中,对于治疗多种细菌性脑膜炎有相当疗效。但是,由于氯霉素对造血系统的毒性颇为严重,因此监测体内氯霉素的浓度,可以指导临床安全用药,增强疗效,避免和减少毒副反应。 高效液相色谱法测定体内药物浓度,方法专一可靠,分析简便快速,国外近年来有应用于体液中氯霉素测定的报道。本文建立的测定人体脑脊液和血液中氯霉素含量的分析方法与上述报道比较,具有样品处理方便、流动相组成简单以及浓度测定线性范围广等特点。

An HPLC method for the determination of chloramphenicol in human plasma and cerebrospinal fluid has been developed. The method is simple and convenient. Samples are only collected . in 100μl. The chlorampenicol concentration can be calculated either by peak height ratio or by peak area ratio. The standard curve is linear over the range from 0.1 to 26.7μg in sample with correlation coefficient of 0.9999. The recovery of chloramphenicol from plasma and cerebrospinal fluid are 93.0±1.2% and 98.0±1.1% respectively. The coefficients of variation of the intra-and inter-assay are within 1.5% and 63% respectively. The minimum detectable quantity is 0.12μg/ ml in sample.