摘要
目的 分析肿瘤微血管密度 (MVD)与膀胱移行细胞癌 (BTCC)预后的关系。方法 收集临床资料齐全的原发的膀胱移行细胞癌组织石蜡标本 6 7例 ,以正常膀胱黏膜上皮 10例作为对照 ,通过免疫组化方法用CD34 显示各级膀胱肿瘤的微血管改变并做计数测得微血管密度 ,并对相应患者的预后情况进行随访。结果 (1)MVD与BTCC的临床分期、病理分级以及预后呈正相关。膀胱癌术后无瘤存活的 31例平均值为 138.2± 35 .9,发生复发或转移的 36例为 196 .6± 5 0 .2 ,差异显著。 (2 )经多因素分析筛选 ,MVD是BTCC的独立预后参数。 (3)对 6 7例BTCC组织标本另以微血管计数值 12 0、2 0 0为界值将相应 6 7例随访患者分为三组 ,以Kaplan -Meier生存曲线对比 ,三组间无瘤生存率差异显著。结论 MVD与BTCC分期、分级以及预后密切相关 ,可以为BTCC预后评估和临床诊治提供参考。
Objective To evaluate the value of mic ro vessel density (MVD) in prognosis of bladder transitional cell carcinoma (BTCC). Methods CD 34 expression was observed by immunohistochemica l tech nique in 67 samples of primary BTCC tissues and 10 normal bladder tissues. Sur vival curves were constructed using the method of Kaplan and Meier. Difference b etween each group was evaluated using the Log Rank test. Independent prognostic factors were evaluated using Cox′s proportional hazards regression model. Results (1)MVD was significantly correlated with clinical stage, patho l ogical grade and prognosis. Mean MVD of 31 cases who survived with carcinoma-fre e was 138.2±35.9, and 36 cases with cancer recurrence and metastasis was 196.6±50.2. There was significant difference between the groups. (2)Multiva riate an alysis showed that MVD was an independent prognostic parameter. (3) 67 cases wer e divided into three groups according MVD value of 120 and 200. The difference of carcinoma-free survival rates was significant among the groups. Conclus ions MVD is an important and independent prognostic factors of BTCC.
