摘要
目的 :探讨间断性低氧对机体免疫反应的影响。方法 :以模拟海拔高度间断性低氧 (4h/d)模型观察大鼠脾淋巴细胞对丝裂原 (ConA)的反应性。结果 :与对照相比 ,5km急性低氧 4h大鼠淋巴细胞的转化率下降 2 5 .4 3% (P <0 .0 5 ) ,间断性低氧 2、5、15d后淋巴细胞转化率分别为 97.0 3%、10 4 .5 %和 99.5 5 % ,与对照组无明显差异。 2km间断性低氧 (4h/d) 1、2、5、15d ,大鼠脾淋巴细胞转化率分别为 93.19%、96 .4 3%、99.0 3%和 10 0 .5 4 % ,与对照组无明显差异。脾单个核细胞DNA含量显示 ,5km急性低氧 4hDNA含量明显下降 (76 .2 2 %± 7.0 6 % ,P <0 .0 5 ) ,间断性低氧 5d和 15d后与对照组无显著差异。结论 :急性低氧抑制淋巴细胞的转化 ,并随低氧的加重而增加 ,重复间断性低氧暴露其抑制作用减弱 ,引起大鼠淋巴细胞转化产生适应。推测免疫适应与HPA轴的适应有关。
Aim: To investigate intermittent hypoxia effects on splenocyte mitogen-induced proliferation.Methods: Rats were exposured to intermittent hypoxia in a hypobaric chamber 4 h/d for 1 d, 2 d, 5 d and 15 d. Results: 5 km(10.8% O 2) hypoxia for 1 d significantly inhibited ConA-induced splenocytes proliferation by -74.57%±7.33%(P<0.05). Hypoxia(5 km) for 2 d、5 d and 15 d did not markedly affect splenocyte proliferation(97.03±7.18%, 104.5%±8.38%, 99.55%±3.8% respectively). Hypoxia 2 km (16.0% O 2) for 1 d, 2 d, 5 d and 15 d had no influence on splenocytes proliferation(93.19%±11.88%, 96.43%±7.9%, 99.03 %±10.97%, 100.54%±9.54% respectively). We also demonstrated that acute hypoxia exposure (5 km) 4 h significantly suppressed DNA contents of rat splenocytes by 76.22%±7.06%(P<0.05). The suppressed DNA synthesis were returned to control level after the hypoxia for 5 d and 15 d. Conclusion: These results suggest that the acute hypoxia(5 km, 4 h) induces a transient suppression on splenic lymphocyte proliferation, and the intermittent hypoxia may induce an adaptation response of the splenocytes proliferation.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第1期5-8,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目 (3 0 0 70 2 89)
关键词
低氧
淋巴细胞转化
适应
hypoxia
splenocyte proliferation
adaptation
