硝苯地平对自发性高血压大鼠血管内皮细胞的保护作用(英文)

Protective effects of nifedipine on endothelial cells in spontaneously hypertensive rats

背景:研究表明,硝苯地平能够改善血管内皮细胞功能,而对于其扩张血管的机制,目前仍在进一步的研究中。目的:观察硝苯地平控释剂对自发性高血压大鼠(SHR)一氧化氮(NO)及诱导性一氧化氮合酶(iNOS)的影响。设计:以实验动物为研究对象的观察对比研究。单位:一所医学院的动物实验中心。材料:本实验于2002-04/2002-05在山东大学医学院动物实验中心完成。实验动物为近交SHR大鼠21只,体质量(300±20)g,由山东大学医学院实验动物中心提供,清洁级。随机分为3组,即对照组、正常剂量组、低剂量组,每组7只。方法:对照组、正常剂量组和低剂量组分别灌胃生理盐水10mL/kg、硝苯地平控释剂(拜新同)溶液(0.3g/L)10mL/kg和3mL/kg,1次/d,连续15d。末次给药后摘眼球取血并取大鼠心、肺分别测定血清NO和iNOS的含量。主要观察指标:各组大鼠的NO含量、iNOS活性比较。结果:灌胃15d后,正常剂量组的NO含量与对照组、低剂量组比较,差异均有显著性意义(P<0.01);正常剂量组心、肺组织块中iNOS活性降低,与对照组和低剂量组比较差异均有显著性意义(P<0.01,P<0.05)。结论:硝苯地平可在降低血压的同时,提高血清NO的含量,并且能对抗血压增高所造成的iNOS的活性增强(或二者互为因果)。

BACKGROUND:Research has proved that nifedipine can improve the functions of vascular endothelial cells.However,it still needs further study on its mechanism on dilating vessels. OBJECTIVE:To observe the effects of controlled-release nifedipine to nitrogen monoxide(NO) and induced nitric oxide synthase(iNOS) in spontaneously hypertensive rats(SHR). DESIGN:Comparative observational study based on experimental animals. SETTING:Experimental animal centre in a medical college. MATERIALS:This study was completed in Experimental Animal Centre of Shandong University during April to May 2002.Twenty-one recombinant inbred rats of clean class with body mass of(300±2) grams were provided by Experimental Animal Centre of Shandong University.They were randomly divided into three groups:control group,normal group and low dose group,each with 7 rats. METHODS:Saline(10 mL/kg),10 mL/kg and 3 mL/kg controlled-release nifedipine solution(0.3 g/L) were poured into stomach to rats in control group,normal dose group and low dose group once everyday for 15 days respectively.Eyeball was removed to collect blood and heart and lung were removed at the same time to test the content of NO and iNOS in serum after last administration. MAIN OUTCOME MEASURES:Content of NO,comparison of iNOS activity between groups. RESULTS:There was significant difference on NO content between normal dose group and control group,low dose group after 15 days' administration(P< 0.01).Compared with control group and low dose group,there was significant difference on the iNOS activity in heart and lung of normal dose group(P< 0.01,P< 0.05). CONCLUSION:While nifedipine reduces the blood pressure,it can also improve the content of NO and resist the increasing activity of iNOS casued by increased blood pressure.