内源性阿片肽参与胎兔窘迫过程的实验研究(英文)

Experimental study of endogenous opioid peptides participating in the fetal distress

背景:内源性阿片肽主要通过μ受体介导,对呼吸及心跳产生抑制作用,从而导致胎儿窘迫的发生、发展。因此,μ受体的介导,是内源性阿片肽参与胎儿窘迫及窒息病理过程中的最重要途径。因此,针对阿片受体专一性较强的拮抗剂β-FNA(β-Funaltrexamine,阿片μ受体拮抗剂)和I-CI174864(阿片δ受体拮抗剂)十分有助于减少胎儿宫内窘迫的发生。目的:从阿片肽角度探讨阿片μ受体及δ受体在胎兔窘迫中的作用。设计:以实验动物为研究对象,完全随机分组,随机对照研究。材料:本实验选取健康雌性新西兰纯种白兔20只,按随机抽签法将兔分为5组,其中16只孕30d母兔以窒息法制成胎兔宫内窘迫模型,迅速剖宫取出胎兔,每窝6～8只。将胎兔分为胎兔窘迫未治疗组(胎窘组,29只)、胎兔窘迫生理盐水治疗组(盐水组,25只)、胎兔窘迫β-FNA(β-Funaltrexamine)治疗组(FNA组,28只)、胎兔窘迫ICI174864治疗组(ICI组,31只)。干预:盐水组、FNA组、ICI组母兔窒息前分别静脉注入生理盐水、阿片μ受体拮抗剂β-FNA及阿片δ受体拮抗剂I-CI174864。另选4只孕30d母兔断颈处死后,剖宫取出胎兔28只作为对照组。以上5组胎兔均于剖宫产后1,5,10,15,30min进行呼吸、心率、肤色、肌张力、神经反射的Apgar评分。主要观察指标:胎兔取并置于恒温箱后1,5,10,15?

BACKGROUND:Endogenous opioid peptides are mainly mediated by μreceptor and produce inhibitory effect on breathing and heartbeat,and because of this,fetal distress happens and develops. Therefore,μreceptor mediation is the most important way that endogenous opioid peptides participate in the fetal distress and the pathological process of suffocation.β-FNA(β-Funaltrexamine,μ-receptor antagonist) and ICI174864(δ-receptor antagonist) are helpful to reduce the happening of fetal distress. OBJECTIVE:To explore the roles of μand δreceptors in the fetal distress based on the point of view that opioid peptides are involved. DESIGN:Completely randomized allocation and randomized and controlled trial using experimental animals as the subjects. MATERIALS:Twenty healthy white New Zealand purebred rabbits were randomly divided into 5 groups,and among them 16 rabbits were pregnant for 30 days and were put to death with the method of suffocation.The fetal rabbits were taken out immediately after cesarean section,with 6-8 fetal rabbits in each den. The fetal rabbits were classified into the following groups:untreated fetal distress group,(fetal distress group, 29 fetal rabbits),normal saline rabbits group(Normal saline group, 25 rabbits),rabbit distress β-FNA group(β-Funaltrexamine )(FNA group,28 rabbits),fetal rabbits ICI174864 treatment group(ICI174864 group, 31 rabbits). INTERVENTIONS: Pregnant rabbits in the saline group, FNA group ,ICI group were intravenously given a bolus of normal saline or the opiate antagonist β-FNA or ICI174864 and were then asphyxiated.Another 4 rabbits that were pregnant for 30 days were put to death with the cut on the neck, and 28 fetal rabbits were taken out as the control group. After being delivered by cesarean section, all rabbit fetuses in the above five groups were assessed by Apgar scoring for respiration,heartbeat, skin color, muscle tension,and response to stimulation at 1,5,10,15 and 30 minutes of age. MAIN OUTCOME MEASURES:Apgar scoring for respiration, heartbeat, skin color, muscle tension,and response to stimulation at 1,5,10, 15 and 30 minutes of age was made. RESULTS:The total Apgar score for respiration,heartbeat,skin color,muscle tension in the control group was the highest(8.8±1.1).And that of FNA group was 6.8±1.7,obviously higher than that of the fetal distress group(2.1±1.0) and the saline-treated pups(2.5±1.1) and(t=2.832 and 2.795,P< 0.01).That of group ICI was 4.9±0.7,markedly higher than that of the saline-treated pups and the fetal distress group(t=2.232 and 2.195,P< 0.05).There was no marked difference between the saline group and the distress group (P >0.05) and between the FNA group and the control group(P< 0.05).The total Apgar scores of the ICI group, saline group, and distress group were significantly lower than that of the control group(t=2.913,2.893,P< 0.01 and t=2.174,P< 0.05). CONCLUSION:Endogenous opioid peptides participates in the process of the happening and development of fetal rabbit distress that is mediated by opioid peptides receptor,the effect of μreceptor was much more than that of δreceptor.This study provides a basis for the evaluation of fetal distress and intervening at an earlier stage to enhance prognostic function.