期刊文献+

HepG_2人肝癌细胞胰岛素样生长因子1受体定量分析 被引量:5

Quantitative analysis of IGF1Rs on human hepatocellular HepG_2 cells
下载PDF
导出
摘要 目的 对人肝癌HepG2 细胞上的胰岛素样生长因子 1受体 (type 1receptorofinsulin likegrowthfactor ,IGF1R)进行定量分析。方法 常规培养HepG2 细胞 ,以Iodogen法 13 1I标记抗IGF1R单克隆抗体 1H7,SephadexG 5 0纯化标记反应液 ,13 1I 1H7和非标记 1H7与HepG2 细胞表面的IGF1R竞争结合 ,Scatchard法分析HepG2 细胞上IGF1R的平均表达量。结果 每孔HepG2 细胞 (2 0× 10 5)表面IGF1R的Bmax为 6 746× 10 -12 mol/L ,平均单个HepG2 细胞IGF1R的密度为 2 .0 3×10 4/细胞。结论 本实验条件下制备的 13 1I 1H7满足作为IGF1R特异性配体的要求 ,碘标记 1H7对其与IGF1R结合活性无明显影响 ,HepG2 细胞表达高密度与高亲和力的IGF1R。 Objective To quantitatively analyze type 1 receptor of insulin-like growth factors (IGF1Rs) on the HepG 2 cells. Methods 1H7 (monoclonal antibody against IGF1R) was labeled with radionuclide 131 I by Iodogen method. The mixture was purified with Sephadex G-50 column, 131 I-1H7, and its unlabeled counterpart 1H7 competitively bound to IGF1Rs on the HepG 2 cells. The average number of IGF1R on the HepG 2 cells was analyzed by Scatchard method. Results The Bmax of HepG 2 cells (2.0×10 5) in every well was 6.746×10 -12 mol/L. The expression of IGF1R on the HepG 2 cells was 2.03×10 4/cell. Conclusion IGF1Rs are expressed on the HepG 2 cells with high density and high affinity, which may be used in other related studies.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2005年第3期206-208,共3页 Journal of Third Military Medical University
关键词 HEPG-2细胞 IGF1R 定量分析 HepG 2 cells IGF1R quantitative analysis
  • 相关文献

参考文献9

  • 1苗积生,沈毅.放射性核素标记的单克隆抗体的定量测定[J].上海医学,1999,22(8):457-459. 被引量:2
  • 2Hatada E N, McClain D A, Potter E, et al. Effects of growth and insulin treatment on the levels of insulin receptors and their mRNA in Hep G2 cells[J]. J Biol Chem, 1989, 264(12): 6741-6747.
  • 3Kosaki A, Webster N J. Effect of dexamethasone on the alternative splicing of the insulin receptor mRNA and insulin action in HepG2 hepatoma cells[J]. J Biol Chem, 1993, 268(29): 21990-21996.
  • 4Levy J R, Krystal G, Glickman P, et al. Effects of media conditions, insulin, and dexamethasone on insulin-receptor mRNA and promoter activity in HepG2 cells[J]. Diabetes, 1991, 40(1): 58-65.
  • 5Ouban A, Muraca P, Yeatman T, et al. Expression and distribution of insulin-like growth factor-1 receptor in human carcinomas[J]. Hum Pathol, 2003, 34(8): 803-808.
  • 6Pezzino V, Milazzo G, Frittitta L, et al. Radioimmunoassay for human insulin-like growth factor-I receptor: applicability to breast carcinoma specimens and cell lines[J]. Metabolism, 1991, 40(8): 861-865.
  • 7Santos A, Yusta B, Fernandez Moreno M D, et al. Expression of insulin-like growth factor-I (IGF-I) receptor gene in rat brain and liver during development and in regenerating adult rat liver[J]. Mol Cell Endocrinol, 1994, 101(1-2): 85-93.
  • 8Navab R, Chevet E, Authier F, et al. Inhibition of endosomal insulin-like growth factor-I processing by cysteine proteinase inhibitors blocks receptor-mediated functions[J]. J Biol Chem, 2001, 276(17): 13644-13649.
  • 9Zarrilli R, Pignata S, Romano M, et al. Expression of insulin-like growth factor (IGF)-II and IGF-I receptor during proliferation and differentiation of CaCo-2 human colon carcinoma cells[J]. Cell Growth Differ, 1994, 5(10): 1085-1091.

二级参考文献4

共引文献1

同被引文献28

  • 1李宝江,朱志华,王军业,侯景辉,赵进明,张蓬原,姚广裕,王曦,龙浩,杨名添,戎铁.Ki67、P53、VEGF和C-erbB-2在乳腺癌组织中表达的相关性研究及其临床意义[J].癌症,2004,23(10):1176-1179. 被引量:126
  • 2任均田,李峰,谢春霖,迟文.Iodogen法碘标记McAb技术探讨[J].放射免疫学杂志,1996,9(3):145-146. 被引量:4
  • 3杨福兵,刘剑波,陈礼刚,董劲虎,黄云华,王新志,胡殿峰.脑胶质瘤患者血清IGFBP-Ⅱ表达及临床意义[J].中华神经外科疾病研究杂志,2006,5(4):365-366. 被引量:5
  • 4Liu M, Lawson G, Delos M, et al. Predictive value of the frac- tion of cancer cells immunolabeled for proliferating cell nuclear antigen or Ki67 in biopsies of head and neck carcinomas to identify lymph node metastasis: comparison with clinical and radiologic examinations[J]. Head Neck, 2003,25 (4) : 280-288.
  • 5Klintman M, Bendahi PO, Grabau D, et al. The prognostic value of Ki67 is dependent on estrogen receptor status and his- tological grade in premenopausal patients with node-negative breast cancer[J]. Mod Pathol,2010,23(2):251-259.
  • 6Key TJ, Appleby PN, Reeves GK, et al. Insulin-like growth factor 1 (IGF1), IGF binding protein 3 (IGFBP3), and breast cancer risk: pooled individual data analysis of 17 prospective studies[J]. Lancet Oneol, 2010,11 (6) : 530-542.
  • 7Pavelic J, Pavelic L, Karadza J, et al. Insulin like growth factor family and combined antisense approach in therapy of lung car- cinorna[J]. Mol Med,2002,8(3) : 149-157.
  • 8Zumkeller W. IGFs and IGFBPs: surrogate markers for diagno- sis and surveillance of tumour growth[J]? Mol Pathol, 2001, 54(5) :285-288.
  • 9Valentinis B, Baserga R. IGF-1 receptor signalling in transfor- mation and differentiation [J]. Mol Pathol, 2001,54 (3):133- 137.
  • 10Kaaks R, Toniolo P, Akhmedkhanov A, et al. Serum C-peptide, insulin-like growth factor(IGF) -I, IGF-binding proteins, and color- ectal Cancer risk in women. J Natl cancer Inst, 2000,92 (19): 1592-600.

引证文献5

二级引证文献20

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部