钙调素mRNA和蛋白在非小细胞肺癌中的表达及其临床意义

Expression of calmodulin and its clinical significance in non-small cell lung cancer

目的 探讨钙调素 (CaM)在非小细胞肺癌 (NSCLC)中的表达水平及其临床意义。方法 采用原位杂交及免疫组织化学技术检测CaM在 64例非小细胞肺癌标本中的表达。结果 在NSCLC中CaMmRNA及CaM蛋白表达检测结果基本一致。CaMmRNA的阳性表达率为 60 4% (3 9/64 ) ,而CaM蛋白为 65 6% (4 2 /64 ) ;低分化的肿瘤标本中CaMmRNA阳性表达率 (70 0 % ,2 1/3 0 )显著高于中高分化的肿瘤标本 (4 5 3 % ,15 /3 4) ;Ⅲ～Ⅳ期CaMmRNA阳性表达率 (81 8% ,2 7/3 3 )显著高于Ⅰ～Ⅱ期 (5 1 6% ,16/3 1) ;有淋巴结转移的CaMmRNA阳性表达率 (64 0 % ,2 5 /3 9)显著高于无淋巴结转移的肿瘤标本 (2 8 0 % ,7/2 5 )。CaM的表达与患者性别、年龄及组织学类型无明显关系。结论 CaM在非小细胞肺癌中有着较高的表达率 ,且与肿瘤的分化程度、临床分期及淋巴结转移有一定的关系 ,提示钙调素表达的增高可能在促进肺癌的浸润转移中起重要作用。

Objective To explore the expression level of calmodulin (CaM) in non-small cell lung carcinoma (NSCLC) and its clinical significance. Methods The expression levels of CaM and CaM mRNA were detected in 64 human NSCLC samples by immunohistochemistry and in situ hybridization, respectively. Results The positive rates of CaM mRNA and CaM protein were 60.4% and 65.6%, respectively. The positive rates of CaM mRNA in low-differentiated samples were significantly higher (70.0%) than those in moderate- to well-differentiated samples (45.3%). The expression rates of CaM in Ⅲ-Ⅳ stages (81.8%) were significantly higher than those inⅠ-Ⅱstages (51.6%) (P<0.05). The expression rate of CaM in lymph node metastasis (64.0%) was significantly higher than that in non-lymph node metastasis (28.0%) (P<0.05). The positive expression of CaM was not associated with sex and age of patients and the different tissue types of NSCLC. Conclusion High expression of CaM exists in NSCLC and it may be associated with cell differentiation and tumor-node-metastasis (TNM) stages and lymph node status of tumor. The results suggest that high expression of CaM may play an important role in the invasion and metastasis of lung cancer.