反义annexinⅡ对人肺腺癌细胞系SPC-A-1裸鼠移植瘤生长的抑制作用

Effects of annexin Ⅱ antisense vector on the growth of transplanted tumor in nude mice

目的 分析annexinⅡ基因反义表达载体对裸鼠移植瘤生长的影响。方法 在裸鼠皮下分别接种SPC A 1(亲代组 )和SPC A 1 annexinⅡ(反义组 )细胞 ,测定成瘤时间、肿瘤体积及重量 ,显微镜下观察肿瘤细胞及其对周围组织的影响。结果 成瘤时间反义组明显晚于亲代组。反义组接种后形成的肿瘤体积、重量均显著低于亲代组 ,且无一例出现局部侵犯 ,而亲代组则有 2例侵犯周围组织。结论 annexinⅡ基因在促进肿瘤细胞生长及对周围组织的侵袭方面具有重要作用。

Objective To analyze the effects of annexin Ⅱ antisense expression vector on the growth of the transplanted tumor in nude mice. Methods The SPC-A-1 cells (parental group) and SPC-A-1-annexin Ⅱ cells (antisense group) were inoculated subcutaneously in nude mice respectively. The forming time, volume, and weight of tumor were measured. The tumor cells and the surrounding tissues were observed microscopically. Results The forming time of tumor in the antisense group was later than that in the parental group. The volume and weight of tumor in the antisense group were lower than those in the parental group. Furthermore, invasion of tumor in the surrounding tissues was found in 2 cases in the parental group, but not in the antisense group. Conclusion The annexin Ⅱ gene may be important in promoting tumor cell growth and invading the surrounding tissues.