入肝血流阻断对肝硬变大鼠肝脏能量代谢的影响

Effects of temporary hepatic inflow occlusion on hepatic energy metabolism of rats with liver cirrhosis

目的 探讨选择性入肝血流阻断对肝硬变大鼠肝脏能量代谢的影响。方法 观察门静脉转流与非门静脉转流条件下阻断入肝血流不同时程后肝脏能量代谢的变化 ,检测指标包括肝细胞线粒体呼吸控制率 (repiratorycontroara tio ,RCR)、肝组织三磷酸腺苷 (adenosinetirphosphate ,ATP)含量、动脉血酮体比值 (arterialbloodketonebodyratio ,AKBR) ,并与假手术组对照。结果 缺血后肝组织ATP、RCR及AKBR均明显下降 (P <0 0 5 )。再灌注后上述指标逐惭恢复 ,但随缺血时间延长 ,升高幅度明显降低 ,甚至难以恢复。结论 选择性入肝血流阻断对肝硬变大鼠肝脏能量代谢的改变与恢复过程有积极作用。

Objective To investigate the effects of temporary hepatic inflow occlusion (HIO) on hepatic energy metabolism of rats with liver cirrhosis. Methods Animal survival rate and hepatic energy metabolism were observed following different periods of HIO with the effect of portal vein blood bypass (PBB) and without PBB, including repiratory controa ratio (RCR), adenosine tirphosphate (ATP), and arterial blood ketone body ratio (AKBR). The sham group served as the control. Results ATP, RCR and AKBR decreased significantly ischemia (P<0.05). These parameters increased after reperfusion, but the scale descended obviously following the prolongation of ischemia. Conclusion Temporary hepatic inflow occlusion plays an important role in restoration of hepatic energy metabolism.