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Smad在牙本质涎磷蛋白基因表达中的作用

Role of Smad proteins in Transcriptional Regulation of Dentin Sialophosphoprotein Mediated by TGF-β1.
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摘要 目的:研究Smad蛋白在牙本质涎磷蛋白(dentinsialophosphoprotein,DSPP)基因表达中的作用。方法:细 胞培养,瞬时转染和报告基因检测。结果:转化生长因子β1(transforminggrowthfactor-β1,TGF-β1)抑制DSPP 基因启动子活性。过表达野生型Smad3蛋白显著增强了TGF-β1对DSPP基因表达的转录抑制,而过表达Smad3 突变型蛋白则显著抑制了TGF-β1对DSPP基因启动子活性的影响。过表达Smad2野生型或突变型蛋白对TGF -β1抑制DSPP基因启动子活性无明显影响。结论:Smad3在介导TGF-b1对DSPP基因的转录调控中发挥重要 的作用。 Objective: To characterize the role of Smad proteins in transcriptional regulation of dentin sialophosphoprotein (DSPP). Methods: Cell culture, transient transfection and luciferase assay. Results: Transforming growth factor-β1 (TGF-β1) inhibited the activity of DSPP promoter luciferase reporter construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene. Overexpression of wild-type Smad3 potentiated the inhibitory effect of TGF-β1 on transcriptional regulation of DSPP, while dominant negative Smad3 decreased the effect. In contrast to Smad3, wild-type Smad2 or its dominant negative mutant had little effect on TGF-β1 regulation on the promoter activity of DSPP. Conclusions: The results indicated that the crucial role of Smad3 in mediating transcriptional regulation of DSPP by TGF-β1.
作者 何文喜 牛忠英 赵守亮 金卫林 高杰 李萍
机构地区 第四军医大学口腔医学院牙体科 解放军 第四军医大学基础部神经生物研究所
出处 《口腔医学研究》 CAS CSCD 2005年第1期1-3,共3页 Journal of Oral Science Research
基金 国家自然科学基金资助项目(编号:30200315)
关键词 牙本质涎磷蛋白 Smad转化生长因子β1 基因表达 Dentin sialophophoprotein Smad Transforming growth factor-β1 Gene expression
分类号 R78 [医药卫生—口腔医学]
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参考文献10

  • 1何文喜,牛忠英,赵守亮,金卫林,高杰,赵书芳.小鼠牙本质涎磷蛋白启动子报告基因载体的构建和启动子活性分析[J].牙体牙髓牙周病学杂志,2004,14(7):373-377. 被引量：7
  • 2何文喜 牛忠英 赵守亮 等.TGF-β1调控小鼠MDPC-23细胞Smads分子的表达与定位.中国口腔医学研究,2002,1(2):90-92.
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二级参考文献10

  • 1Seenath T,Thyagarajan T,Hall Bet al.Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis iimperfecta type Ⅲ[J].J Biol Chem,2003,278(27):24874
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  • 10何文喜,刘晓爱,牛忠英,赵守亮.转化生长因子β信号转导与成牙本质细胞分化[J].牙体牙髓牙周病学杂志,2002,12(1):44-47. 被引量：8

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口腔医学研究
2005年 第1期

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