频繁摄食蔗糖对口腔牙菌斑钙、磷、氟储库和龋病相关性的研究

The Relationship Between the Mineral Content of Dental Plaque Formed in the Frequent Sucrose Exposure and Enamel Demineralization in 7-days.

目的:观察不同摄食蔗糖频率对菌斑钙、磷、氟含量变化,及对菌斑下釉质脱矿的影响,评价菌斑钙、磷、氟 储库和龋病的相关性。方法:选取10名志愿者,制作附有3块4mm×4mm×1mm大小釉质块的口内装置,在7天 周期培养菌斑的同时,以0次/d～8次/d不同频率滴加20%的蔗糖溶液,每次作用5min,观察7d后菌斑钙、磷、氟 含量、菌斑重量和釉质脱矿。结果:菌斑钙、磷含量随摄糖频率的升高显著下降(P<0.001),氟含量仅在8次/d频 率时较基线有显著降低(P<0.01),而菌斑重量则显著增加(P<0.001),4次/d和8次/d组釉质块出现脱矿,脱矿 釉质块数目、程度与摄糖频率呈正相关。结论:频繁摄糖减少了菌斑矿物质含量,使其缓冲能力下降,并增加了菌 斑的致龋性。

Objective: To measure the effect of frequent sucrose exposure on the content of calcium(Ca), inorganic phosphous(p), fluoride(F) of dental plaque and enamel demineralization. Evaluate the relationship between mineral content of dental plaque and caries. Methods: An acrylic resin appliance containing 3 human enamel blocks (4 mm×4 mm×1 mm) was constructed for each volunteer (total 10). A 20% sucrose solution was dripped onto the enamel blocks from 0~8 times/days in 7-days. After 7-days, we evaluate the content of Ca, P, F, weight of dental plaque and enamel demineralization. Results: The content of Ca, P in dental plaque decreased significantly from 0～8times/days, but F concentration only decreased significantly in 8 times/days comparing the baseline, the wet weight of dental plaque increased sharply. (P<0.001). Enamel demineralization can be found from 4~8 times/days. Conclusions: Frequent sucrose exposure reduced the mineral content and buffer capacity of dental plaque, and increased cariogenicity of dental plaque.