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肿瘤坏死因子相关凋亡诱导配体在外周血单个核细胞中的表达

Expression of TNF related apoptosis inducing ligand in peripheral blood mononuclear cells
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摘要 目的 :研究肿瘤坏死因子相关凋亡诱导配体 (TRAIL)在外周血单个核细胞 (PBMC)中的表达及其意义。方法 :用免疫组织化学的方法检测PBMC中TRAIL的表达情况。结果 :淋巴细胞分离液得到的PBMC中 ,淋巴细胞占 (97.4 8± 3.4 3) % ,单核细胞占 (3.15± 0 .83) % ,TRAIL免疫反应阳性的淋巴细胞占总数的 (2 6 .31± 3.18) % ,呈TRAIL免疫反应阳性的单核细胞占总数的(1.0 4± 0 .13) % ,TRAIL免疫反应阳性物质分布于胞质内 ,核呈阴性反应。结论 :正常人外周血分离的淋巴细胞及单核细胞有TRAIL表达。 Objective: To investigate the expressi on of TNF related apoptosis inducing ligand (TRAIL) in peripheral blood mononuclear cells. Methods: The PBMCs were obtained and purifed from healthy volunteers.Immunohistochemical staining was used to detect the TRAIL immunoreactive expression in PBMCs. Results: Some lymphocytes and monocytes in PBMCs showed TRAIL in immunoreactivity.The immunoreactive positive substance distributed in cytoplasma with negative nuclei. The rate of lymphocytes and monocytes with TRAIL immunoreactivity were (26.31±3.18)% and (1.04±0.13)%,respectively. Conclusion: These results suggest that TRAIL protein may be expressed in some lymphocytes and monocytes in PBMCs.
作者 史治宙 宋朝君 黄威权 金伯泉 付建芳 姬秋和
机构地区 第四军医大学西京医院内分泌科 第四军医大学西京医院免疫学教研室 第四军医大学组织学与胚胎学教研室
出处 《解剖学杂志》 CAS CSCD 北大核心 2005年第1期29-30,i004,共3页 Chinese Journal of Anatomy
关键词 TRAIL 表达 淋巴细胞 肿瘤坏死因子相关凋亡诱导配体 PBMC 外周血单个核细胞 单核细胞 总数 结论 目的 TNF related apoptosis inducing ligand peripheral blood mononuclear cells immunohistochemitry
分类号 R392 [医药卫生—免疫学] R735 [医药卫生—肿瘤]
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参考文献8

  • 1季军捷,卫立辛,李晓东,吴孟超,郭亚军.激活淋巴细胞中TRAIL及其受体表达的初步研究[J].第二军医大学学报,2002,23(7):788-790. 被引量：2
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二级参考文献8

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解剖学杂志
2005年 第1期

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