睡眠剥夺对大鼠胃运动及相关胃肠激素影响的研究

Experimental study of sleep deprivation on gastric motility and the effect of some factors

目的 观察睡眠剥夺对胃排空、胃电活动及降钙素基因相关肽(CGRP)和一氧化氮 (NO)的影响。方法 将 192只SD大鼠随机均分为 3组 ,即急性睡眠剥夺组 (A组 )、慢性睡眠剥夺组 (B组 )、空白对照组 (C组 ) ;每组又随机均分为 2个亚组 ,每亚组 32只大鼠 ,即AI、AII,BI、BII,CI、CII;每个亚组再随平均分为 4个小组 ,每小组 8只大鼠。利用放射分析法 ,使用放射性核素锝 (99mTc) ,采取液相胃排空的方法检测胃的排空 ,利用浆膜法检测胃电的变化 ;利用生化法和放免分析法测定大鼠血浆中的CGRP、NO含量。结果 急性睡眠剥夺导致胃排空速率改变 ,随着睡眠剥夺时间的延长 ,胃的排空速率逐渐降低 ;胃电活动在餐前逐渐降低 ,而餐后无明显规律 ,呈现紊乱状态 ;随着睡眠剥夺时间的延长血浆中CGRP的水平 ,逐渐降低 ,而NO水平却逐渐升高。慢性睡眠剥夺导致胃排空速率降低 ,但随着睡眠剥夺时间的延长 ,胃的排空速率未再发生更为明显的变化 ,呈现低排空状态 ;胃电活动在餐前、餐后均无明显规律 ,呈现紊乱状态 ;血浆中CGRP的水平随着睡眠剥夺时间的延长 ,呈现低水平状态 ,但与干预时间的关系不明显 ;NO水平呈现相对较高状态 ,且与干预时间关系不明显。结论 睡眠剥夺对胃运动有较为明显的影响。

Objective To observe the effect of gastric motility and gastric electric activity on sleep deprivation and the changes of calcitonin gene-related peptide(CGRP) and nitro oxygen(NO). Methods The 192 SD rats were divided into three groups randomly. Gastric emptying was analyzed with radioimmunoassay, using nuclide 99m Tc and gastric emptying of liquids and EGG with the serosa method .By using the immuno-chemistry and chemistry analysis methods, we observed the changes of some connective factors, for example, CGRP and NO. Results The acute deprivation can cause the change of gastric emptying and which is decreased with the time of deprivation, in the meantime, we found the gastric electric activity would be decreased before food, otherwise, the gastric electric activity is irregulary and the gut hormone, for example, CGRP and NO have been changed in the plastic of rats after acute deprivation. In the study we found the gastric emptying have a change obviously in the short time. But with the time of deprivation the gastric empting no have change. And the gastric similar to this, the gut hormone no any change. Conclusions The acute sleep deprivation caused the change of gastric emptying and the change of gastric electric activity obviously. In plastic, the level of CGRP and NO have a change after sleep deprivation. The chronic sleep deprivation caused the change of gastric emptying and the change of gastric electric activity, and caused the function of gastric motility disturbing, and the effect is prolong after chronic sleep deprivation.