高+Gx过载对猴肾脏组织病理学的影响

Histopathological changes in kidneys caused by hypergravity in Rhesus macaque

目的 观察高 +Gx过载作用对猴肾脏组织病理学的影响。方法 9只雄性恒河猴 ,随机分为 4组。对照组与实验 1、2、3组经受 +Gx及其作用时间分别为 +1Gx、30 0s,+15Gx、2 0 0s,+18Gx、16 5s,+2 1Gx、14 0s。使用专用动物离心机 ,加载后的动物即刻处死、取材、制片、染色。用组织病理学方法观察猴肾脏组织学变化。结果 对照组肾脏组织结构未见明显异常。实验组肾小球充血、间质血管扩张、红细胞渗出 ,肾小管上皮细胞高度肿胀 ,胞浆向管腔面破裂。结论 高 +Gx过载作用可造成肾脏组织病理损伤 ,其损伤程度与+Gx值有明显的量 效关系。

Objective To observe the histopathological changes in the kidneys with stress reaction under +Gx loads in rhesus monkeys. Methods Nine male Rhesus macaques monkeys were randomly divided into four groups: control group animals were exposed to +1Gx/300s overload, and experimental groups 1, 2 and 3 animals were exposed to +15Gx/200s, +21Gx/165s and +21Gx/140s (3 animals in each group), respectively. Renal tissue was fixed with 10% buffered formaldehyde and paraffin sectioned, and histopathological changes were observed with microscope. Results In the experimental groups, glomerular congestion, and dilation of interstitial vessels with erythrocytic extravasation were found in both kidneys. Obvious cloudy swelling of the epithelial cells was also noted in the renal tubules. The degree of renal damage was significantly correlated with the increase in +Gx loads. There was no obvious change in the control group. Conclusion Overload gravity can induce significant injury to the kidney in the monkeys. There is a significant correlation of the degree of renal damage with the level of +Gx load.