摘要
探讨cPKCs特定亚型在脑低氧预适应形成过程中的作用 ,借助已建立的小鼠整体低氧预适应模型 ,应用SDS PAGE和Westernblot等生化技术 ,并结合GelDoc成像系统 ,半定量检测脑组织内cPKCα和γ的膜转位水平和蛋白表达量。结果发现 ,随低氧暴露次数 (低氧 2～ 4次 )增加 ,在小鼠海马和皮层组织内cPKCγ膜转位水平增高显著(P <0.0 5 ,n =6 ) ;然而 ,cPKCα的膜转位水平和cPKCγ及γ的蛋白表达量的变化均不显著。提示 。
To explore the role of conventional protein kinases C (cPKCs) in the onset of cerebral hypoxic preconditioning, the biochemistry techniques of SDS-PAGE, Western blot and Gel Doc imagine system were applied to semi-quantitatively analyze the effect of repetitive hypoxic exposure (H1-H4) on the level of cPKCα and γ membrane translocation and protein expression in the brain of mice. Results showed that the level of cPKCγ membrane translocation was enhanced significantly ( *P<0.05, n=6) in response to the repetitive hypoxic exposure both in hippocampi[H0: 100% vs H1-H4: (119.2±7.0)%, (139.3±7.4)% *, (134.2±8.95)% *, (184.0±10.8)% **] and cortex [H0: 100% vs H1-H4: (129.7±13.8)%, (143.3±13.9)% *, (204.0±12.1)% **,(229.5±14.6)% **] of mice. But there was no significant change to be found for the level of cPKCα membrane translocation, and cPKCα and γ protein expression both in hippocampi and cortex. These results suggested that the activation of cPKCγ might be involved in the onset of cerebral hypoxic preconditioning.
出处
《基础医学与临床》
CSCD
北大核心
2005年第1期30-34,共5页
Basic and Clinical Medicine
基金
国家自然科学基金 (30 2 70 5 0 8)
北京市教委科技发展计划项目 (2 0 0 2KJ0 80
KM2 0 0 310 0 2 5 10 0 )
北京市自然科学基金 (70 32 0 0 5 )
北京市优秀人才专项
国家人事部留学回国人员科技活动择优资助项目
教育部优秀青年教师资助项目
