摘要
采用RT-PCR方法扩增腔上囊病病毒(IBDV)VP2 基因,亚克隆到 pAdlox转移载体,与野生型腺病毒ψ5一起共转染Cre8细胞,将IBDV-VP2基因在5型腺病毒中进行表达。结果,获得了含有腔上囊病病毒 VP2 基因的重组腺病毒 Adv VP2,感染重组腺病毒的细胞经 Western blotting检测,得到了1个约37 ku的VP2多肽,而感染空载体病毒的细胞及对照细胞则无此蛋白条带。证实,成功构建了表达IBDV VP2基因的重组腺病毒。
The recombinant plasmid pAdlox VP2 was obtained after the VP2 gene of infectious bursal disease virus (IBDV) cloned by RT-PCR was sub-cloned into the transfer vector pAdlox. The pAdlox VP2 was transfered into Cre8 cells together with the wild-type adenovirus ψ5 DNA to express the VP2 gene of IBDV. The result demonstrated that the recombinant adenovirus containing the IBDV VP2 gene was (constructed) successfully, and a 37 ku protein was expressed successfuly in the Cre8 cells, which was (confirmed) by Western-blotting method.
出处
《中国兽医科技》
CSCD
北大核心
2005年第2期121-123,共3页
Chinese Journal of Veterinary Science and Technology
