胃癌组织Angiopoietin-2及MMP-9表达的意义

Expression of Angiopoietin-2 and Matrix metalloproteinase-9 in gastric carcinoma

目的:观察血管生成素-2(angiopoietin-2)和金属基质蛋白酶-9(MMP-9)在胃癌组织中的表达,并探讨其临床意义. 方法:用免疫组化染色法检测了50例胃癌患者癌组织, 10例非癌胃黏膜组织中angiopoietin-2(Ang-2),MMP-9, CD-34蛋白的表达. 结果:胃癌组织中Ang-2,MMP-9的阳性率分别为56% 和60%,显著高于非癌胃黏膜.他们的表达水平与胃癌的分化程度呈负相关(25% vs 71%,X2=9.18,P<0.01; 31% vs 74%,X2=8.10,P<0.01),与肿瘤的TNM分期(30% vs 78%,P<0.01:35% vs 81%,P<0.01),淋巴结转移(39% vs 70%,P<0.05;43% vs 74%,P<0.05),微血管密度(Ang-2:74.9±11.8 vs 65.6±11.5,P<0.05)成正相关(此外,MMP-9的表达水平还与肿瘤浸润的深度成正相关(33% vs 79%,X2=10.73,P<0.01).Ang-2、MMP-9 在胃癌中的阳性表达率之间存在一定相关性(X2=3.0, P<0.01). 结论:Ang-2、MMP-9在胃癌的侵袭与转移中起重要作用,并且他们之间存在相互诱导或是协同效应,其共位表达可作为判断预后的指标.

AIM: To investigate the expression of angiopoietin-2 (Ang-2) and matrix metalloproteinase-9 (MMP-9) in gastric carcinoma. METHODS: The expression of Ang-2 and MMP-9 was detected in tissues of gastric cancer (n = 50) and non-cancerous gastric mucosa (n = 10) by immunohistochemistry. RESULTS: The positive rates of Ang-2 and MMP-9 in gastric cancer were 56% and 60%, which were significantly higher than those in non-cancerous mucosa. The level of Ang-2 and MMP-9 expression had a negative correlation with the differentiation degree (25% vs 71 %, x2 = 9.18, P<0.01; 31 % vs 74%, x2 = 8.10, P<0.01), but a positive correlation with TNM stage (30% vs 78%, P<0.01; 35% vs 81%, P<0.01), lymphatic metastasis (39% vs70%, P<0.05; 43% vs 74%, P<0.05), or microvascular density (Ang-2: 74.9±11.8 vs 65.6±11.5, P<0.05). In addition, the level of MMP-9 expression was correlated with the invasion depth (33% vs 79%, P<0.01). Also, there was a significant correlation between the expression of Ang-2 and MMP-9 (x2 = 13.0, P<0.01). CONCLUSION: Ang-2 and MMP-9 play a synergistic role in the invasion and metastasis of gastric carcinoma. Their colocalization may be a useful indicator for the prognosis of the disease.