DBP、DEHP及其代谢物MBP、MEHP的体内雌激素样活性分析

Potential Estrogenic Activities of DBP,DEHP and Their Metabolites in Immature Rats Using the Uterotrophic Assay

[目的 ]探讨环境内分泌干扰物邻苯二甲酸二丁酯 (DBP)和邻苯二甲酸 2 乙基己基酯 (DEHP)及其代谢产物邻苯二甲酸单丁酯 (MBP)和邻苯二甲酸 单 乙基己基酯 (MEHP)的雌激素样活性。 [方法 ]按照经济合作与发展组织 (Or ganizationforEconomicCooperationandDevelopment ,OECD)推荐的标准试验方法 ,采用背部皮下注射的方式对出生后 18d雌性未成熟大鼠连续给药 3d ,末次给药 2 4h后处死 ,观察化学物对大鼠体重、子宫重量、滤纸吸干后子宫重和子宫粘液重量等相关指标的变化。 [结果 ]虽然未观察到DBP、MBP、DEHP及MEHP有明显的促子宫增重作用 ,但MBP和MEHP对未成熟雌性大鼠的毒性大于DBP和DEHP ,40～ 10 0 0mg/kgMBP和 10 0 0mg/kgMEHP可减缓大鼠的体重增长速度 ,2 0 0和10 0 0mg/kgMEHP则可明显降低大鼠子宫的粘液分泌量。 [结论 ]DBP、DEHP及其代谢产物MBP和MEHP均无雌激素样活性 ,MBP可减缓大鼠体重增长速度 。

Objective To study the potential estrogenic activities of DBP,DEHP and their metabolites(main toxicants) MBP and MEHP. The standard testing method recommended by OECD was used. PND18 day immature female rats were administrated by subcutaneous injection daily for 3 days. Those rats were humanly sacrificed 24 hr after the last administration and indexes such as absolute uterine weight,blotted uterine weight,etc were determined. There had no statistically significant increase in uterine weight of rats treated with DBP,MBP,DEHP or MEHP. The toxicity of metabolites(MBP and MEHP) was higher than their prototype(DBP and DEHP). 40～1 000 mg/kg MBP and 1 000 mg/kg MEHP could affect the rats' body weight gain. 200 mg/kg and 1 000 mg/kg MEHP could decrease the excreting process of the rats' uterus. [Conclusion] DBP,DEHP and their metabolites MBP and MEHP had no estrogenic activities in immature rats. MBP could affect the rats' body weight gain while MEHP could inhibit the function and development of rats' uterus.