过氯酸铵致肺纤维化作用的实验研究

Explorating the Effects of Ammonium Perchlorate on Pulmonary Fibrosis

[目的 ]探讨过氯酸铵 (AP)致动物肺纤维化作用。 [方法 ]①小鼠胚胎肺成纤维细胞染毒AP 48h后测定羟脯氨酸 (HYP)含量 ;②大鼠经气管内注入AP染毒 7～ 2 9d ,取大鼠肺泡灌洗液 (BALF)和肺组织 ,测定肿瘤坏死因子 α(TNF α)、丙二醛 (MDA)、HYP和胶原蛋白 (Collagen)的含量 ,并对肺组织进行病理检查。 [结果 ]①病理结果显示AP能引起肺急性炎症反应 ,但没有明显的肺纤维化病变 ;②AP染毒小鼠胚胎肺成纤维细胞HYP含量变化不显著 ;③AP低剂量组TNF α含量显著高于阴性对照组 (P <0 .0 1) ,尤其是低剂量第 2 9d组 ;④AP染毒后第 7d高剂量组MDA、HYP、Collagen含量明显高于阴性对照组 (P <0 .0 1) ,呈接触剂量 效应关系。 [结论 ]AP能引起肺急性炎症反应 ,对TNF α、MDA、HYP和collagen合成有一定影响 ;

Objective To explore whether ammonium perchlorate(AP) could cause animal pulmonary fibrosis. ①To determine the levels of hydroxyproline(HYP) of mouse embryo pulmonary fibroblast 48 h after administering AP.②To determine the levels of tumor necrosis factor-α(TNF-α),propanedialdehyde(MDA),HYP and collagen in BALF or rat lung when rats were injected AP by intratracheal instillation for 7 to 29 d; meanwhile,to make a pathological examination of lung. The pathological examination showed that AP could bring about acute inflammatory reaction,but no obvious pulmonary fibrosis.There was no obvious change in the level of HYP in embryo pulmonary fibroblasts after administering AP.The level of TNF-α in low dose group was higher than that of negative control group(P<0.01) and the highest level was found at 29 d.The levels of MDA,HYP and collagen of high dose group at 7 d were obviously higher than those of negative control group(P<0.01) and there was a relationship between exposure and effect. [Conclusion] AP could bring about acute inflammatory reaction and affect the levels of MDA,HYP and collagen.But this experiment could not yet conclude that AP could cause animal pulmonary fibrosis.