银杏叶片剂对γ射线诱发的小鼠PCE和生殖细胞微核率的影响

Effect of Ginkgo Leaf Preparation on Micronucleus Formation in PCE and Germ Cells by γ-Ray Irradiation of Mice

[目的 ]研究60 Co γ射线照射小鼠后 6d和 12d对骨髓嗜多染红细胞 (PCE)和生殖细胞微核的影响及银杏叶片的纠正作用。 [方法 ]清洁级ICR小鼠 48只随机分为 6组 :6d未辐射组 (阴性对照组 )、辐射组 (阳性对照组 )、银杏组 ;12d未辐射组 (阴性对照组 )、辐射组 (阳性对照组 )、银杏组。每只小鼠全身一次性 5 .0Gy剂量60 Co γ射线照射 ,未辐射组假性辐射。此后每天银杏叶片 10mg灌胃 ,连续 6d或 12d。于未次灌胃后次日 (第 7天和第 13天 )观察小鼠PCE和睾丸生殖细胞微核率。 [结果 ]灌胃 6d ,辐射组PCE和睾丸生殖细胞微核率显著高于未照射组 (P <0 .0 1,P <0 .0 5 ) ,银杏组PCE微核率显著低于辐射对照组 (P <0 .0 5 ) ,但睾丸生殖细胞微核率差异无显著性 (P >0 .0 5 )。灌胃 12d辐射组PCE微核率与未照射组比差异无显著性 (P >0 .0 5 ) ,但显著低于灌胃 6d辐射组 (P <0 .0 5 )。辐射组、银杏组睾丸生殖细胞微核率显著高于未照射组 (P <0 .0 5 ) ,分别与 6d组比有下降但无统计学意义。 [结论 ]辐射后第 7天小鼠PCE、睾丸生殖细胞具有遗传损伤作用 ,银杏叶片具有纠正辐射引起的损伤作用。辐射后第 13天PCE。

Objective The purpose of this study was to evaluate the effect on micronucleus formation in PCE of bone marrow and germ cells by γ-ray irradiation and the anti-irradiation activities of Ginkgo leaf preparation in mice. The mice generally irradiated by 5.0 Gy 60Co-γ-ray,each mouse was given through stomach 10 mg Ginkgo leaf preparation every day for 6 days and 12 days. On the 7 th day and 13 th day,the micronuclei rates of PCE in bone marrow and germ cells were observed. On the 7 th day,the micronuclei rates of PCE in bone marrow and germ cells in irradiated groups were higher than those in non-irradiated groups(P<0.01,P<0.05); the micronuclei rates of PCE in bone marrow in Ginkgo group was lower than those in irradiated group(P<0.05). The differences between the micronuclei rates of germ cells in Ginkgo group and irradiatied group were not statistically significant(P>0.05). On the 13 th day the micronuclei rates of PCE were lower than those on the 7 th day in irradiated group(P<0.05). On the 13 th day the micronuclei rates of germ cells in irradiated group and Ginkgo group were higher than those in non-irradiated group (P<0.05). The differences between the micronuclei rates of germ cells of irradiated group and Ginkgo group on the 7 th and 13 th day were not statistically significant(P>0.05). [Conclusion] There is genetical injury of PCE in mice bone marrow and germ cells after γ-ray irradiation and the proctective effect of Ginkgo leaf preparation on irradiating injury of PCE in bone marrow on the 7 th day. The micronucleus rates of PCE in bone marrow and germ cells in γ-ray irradiatied mice might be related to normal on the 13 th day.