小麦品种和白粉菌相互作用中温度对寄主基因表达的影响

THE INFLUENCE OF TEMPERATURE ON HOST GENE EXPRESSION IN THE INTERACTION OF WHEAT CULTIVARS AND ERYSIPHE GRAMINIS TRITICI

对小麦抗源品种肯贵阿和感病品种绵阳15号杂交的F_1、F_2、F_3家系,于幼苗期接种白粉菌411菌株,并在人工气候箱内培养.结果表明,肯贵阿具有一对显性抗性基因,绵阳15号具一对抑制基因.在14℃下,肯贵阿品种所携的 KG 抗性基因控制着对411菌株的抗性,而感亲的抑制基因不起作用;在20℃下,感亲的抑制基因与抗亲的 KG 基因互作产生抑制作用,致使 KG 抗性基因的显性效应受到抑制.KG 基因不具温敏性.

Powdery milderv resistant wheat cultivar Ken-gui-A was crossed with the susceptible cultivar Mianyang 15 to obtain F_1,F_2 and F_3 lines,and then the seeds were sown.The plants were inoculated with culture 411 of Erysiphe graminis tritici at the seedling stage and put in growth cabinets at 14℃ and 20℃.The results showed that Ken-gui-A contained one pair of dominant resistance genes and Mianyang15 possessed one pair of inhibiting genes.At 14℃,KGgene carried by Ken-gui-A controlled the resistance to 411 culture and the inhibiting gene played no role.At 20℃,the inhibiting gene and resistance gene interact- ed and exhibited an inhibiting effect.This time the dominance effect of KG gene was masked.The experimintal result also showed KG gene does not possess temperature sensi- tive resistance.