摘要
目的 观察累加电针对神经痛大鼠背根神经节 (DRG)中胶质细胞源性神经营养因子 (GDNF)mRNA表达的影响。方法 实验分为 3组,对照组为正常大鼠,神经痛组为大鼠坐骨神经慢性限制性损伤(CCI)致神经痛模型,神经痛 +电针组为术后第 7d起隔日给予神经痛大鼠累加电针治疗。各组动物处死后,取L4 L6 DRG,冰冻切片,采用原位杂交的方法,观察累加电针对神经痛大鼠DRG中GDNFmRNA表达的影响。结果 大鼠坐骨神经结扎后出现显著热痛敏,累加电针能明显抑制神经痛大鼠热痛敏;CCI诱发神经痛后,大鼠DRG中GDNFmRNA表达明显增高,累加电针可以使其进一步增高。结论 实验提示内源性GDNF可能参与累加电针对大鼠神经痛的治疗作用。
Objective To investigate the effect of cumulative electroacupuncture (EA) on the expression of glial cell line-derived neurotrophic factor (GDNF) mRNA in dorsal root ganglions (DRG) of neuropathic pain rats.Methods Rats were randomly divided into three groups: control group, neuropathic pain (NP) group and EA group. Chronic constriction injury (CCI) to sciatic nerve was performed on rats to induce neuropathic pain. On the 7 th day after surgery, rats with hyperalgesia to radiant heat were selected as neuropathic pain rats and one group received cumulative EA treatment once every other day. Expressions of GDNF mRNA in L 4-L 6 DRG of different groups were exmined using in situ hybridization technique.Results The results showed that CCI surgery induced stable and lasting hyperalgesia and cumulative EA had potent analgesia effect on heat hyperalgesia of neropathic pain rats. The expression of GDNF mRNA in DRG was significantly increased during neuropathic pain and could be further enhanced by cumulative EA.Conclusion Our results suggested that endogenous GDNF might be involved in EA analgesia on neuropathic pain of rats.
出处
《上海针灸杂志》
2005年第2期33-36,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金资助(No30472232
30070948)
